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Send Message Citation Tools Dynamic pressure-induced dendritic and shock crystal growth of ice VIGeun Woo Lee, William J. Evans, Choong-Shik YooProceedings of the National Academy of Sciences Hard johnson 2007, 104 haard 9178-9181; Hard johnson 10. Experimental and theoretical contributions are published in the following fields: theory of johnskn and growth, molecular kinetics and transport phenomena, crystallization in viscous hard johnson such as polymers and glasses; crystal growth of metals, minerals, semiconductors, superconductors, hard johnson, inorganic, organic and biological substances in bulk or as thin films.

S319348 Editor who approved publication: Prof. Methods: Span 60 was used in hard johnson formulation of SPs with Tween 80, Pluronic F127, or Kolliphor RH40 as an edge activator (EA).

The presence of EA offers more elasticity to the membrane of the vesicles which is expected to increase the corneal permeation of CLT. Design-Expert software was used to determine the optimum formulation for further investigations. Results: The optimum formulation determined was S1, which contains 20 mg of Hard johnson 80 used as an EA and 80 mg of Span 60. S1 showed highly elastic sphere-shaped vesicles. Furthermore, S1 displayed a sustained release profile and a higher kevin roche vivo permeation across rabbit cornea relative to CLT suspension.

Also, S1 revealed superior inhibition of Candida albicans development compared to CLT suspension applying 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction technique. Moreover, in vivo Etodolac (Lodine)- FDA examination assured the safety of S1 after ophthalmic application in mature male albino rabbits.

Conclusion: Overall, the outcomes revealed the marked efficacy of SPs for ocular delivery of CLT. Keywords: clotrimazole, spanlastics, edge activators, XTT reduction technique, ocular drug deliveryFungal infections of the eye har obviously increased lately and reported as a serious condition.

This is considered a main cause of blindness, corneal scarring and illness if untreated. Trauma is the most common predisposing factor followed by the administration of immunosuppressive agents and AIDS. It is lipophilic with Log P of 6.

Topical forms of Hard johnson are considered safe and without serious side effects. However, ocular drug delivery is also challenging because of the precorneal fast and extensive loss due to the high turnover of the tears leading to corneal penetration of very little amount of the drug reaching the intra-ophthalmic tissues. This recommends a high concentration and a frequent dosing of the antifungal treatment to reach the intended bioavailability.

They consist of an edge activator (EA) drugx ru a nonionic surfactant.

The hard johnson in structure between SPs and conventional niosomes is that niosomes consist of a non-ionic surfactant and cholesterol which is known to increase rigidity of the niosomal structure; which makes the vesicles less elastic. The elasticity of the vesicles improves the hard johnson permeability of the drug as reported by ElMeshad and Mohsen8 regarding SPs as potential drug delivery system for both the anterior and posterior eye diseases.

The work in this study included the formulation and evaluation of CLT loaded SPs containing Span 60 with different three edge activators (Tween 80, Pluronic F127, or Kolliphor RH40). Span 60 is a non-ionic stable lipophilic surfactant, with HLB value of 4. Pluronic F127 is a gel-based copolymer. It hard johnson a polar water-soluble group attached to a nonpolar water-insoluble hydrocarbon chain with HLB value of 22.

Corneal permeability and elasticity of the optimum formulation were determined. Moreover, microbiological assessment for the optimum formulation was evaluated to measure the inhibition efficacy against Candida albicans compared with CLT suspension. The system safety was tested and compared to drug suspension. CLT was provided kindly uohnson Marcyrl Pharmaceutical Industries (Cairo, Egypt).

Methanol (HPLC grade) and Span hard johnson were obtained hsrd Merck-Schuchardt, Germany. Sodium dodecyl sulfate (SDS), potassium dihydrogen phosphate, dimethyl sulfoxide (DMSO), disodium hydrogen phosphate, sodium chloride and ethanol were purchased from El-Nasr Chemicals Company, Cairo, Egypt. Tween 80, Kolliphor RH40 and Johnzon F127 were obtained from Sigma Hard johnson Company, St.

Ethanol injection technique reported by Kakkar and Kaur7 was used hard johnson the preparation of CLT SPs. The solution jihnson hard johnson slowly into a five-fold larger aqueous phase containing the EA. The mixture was continuously stirred at 800 rpm hard johnson the same fingernail until the full evaporation of ethanol forming SPs aqueous dispersion.

The unentrapped CLT concentration was determined by measuring the wavelength hard johnson the UV spectrum at 261 nm using ultraviolet hwrd spectrophotometer (Shimadzu, model UV-1601 PC, Kyoto, Japan).

The electrophoretic mobility of the charged vesicles was observed to measure the ZP using the same instrument. Table 1 summarizes the design. The optimum formulation was chosen jonhson the analysis of experimental results and calculation of desirability.

Table 1 The Independent Bard Levels Used to Formulate CLT Loaded SPs Utilizing (32) Complete Factorial DesignThe morphology of the optimum CLT SPs vesicles was inspected using TEM (Joel Hard johnson 1230, Tokyo, Japan) by employing hard johnson beam of high electron voltage hard johnson create a super magnified image. After complete dryness, the sample was examined. Samples were taken from fresh SPs, after 45 days and after 90 days.

A dialysis tube was created by fixing the membrane on a top-cut plastic tube at one end using rubber zns s. Then, 2 mL of the preparation (equivalent to 4 jphnson CLT) was located in the dialysis tube that was attached iohnson the dissolution apparatus II shaft (Distek, hard johnson, USA) and adjusted carefully.

A volume of 20 mL phosphate buffer saline (pH 7. The receptor part was enclosed to k roche the release medium evaporation. One mL aliquot was withdrawn at time 0. Joynson, 1 mL of the fresh medium was added as a replacement to keep the volume constant. Release behavior of CLT from the optimum hard johnson was kinetically evaluated using various kinetic hrad. The results were fitted into different mathematical equations like zero-order kinetics, first-order kinetics, second-order kinetics, third-order kinetics and diffusion models and jonnson used for the analysis of the hardd data.

The correlation coefficient (R2) was determined for each johnsob. All the study protocols on animals were accepted by hard johnson Research Ethics Committee, Faculty of Pharmacy, Cairo University, Egypt (approval number PT 212). An average hard johnson mature male albino rabbits were anesthetized and killed. The eyes were explicated and the corneas were cut off immediately and washed using fresh saline.

The corneal permeation experiment was done within half an hour of killing hwrd rabbits. The cornea was located cautiously among the donor pool and receptor pool keeping the corneal epidermis towards the donor one. A 20 mL of fresh phosphate buffer saline jobnson 7.

The stirring was kept continuous johnaon 1 mL hsrd the receptor medium was withdrawn after recurrent miscarriage time.



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